An extract on #puppylife
Researchers commonly replicate DNA in vitro using the polymerase chain reaction (PCR). PCR uses a pair of primers to span a target region in template DNA, and then polymerizes partner strands in each direction from these primers using a thermostable DNA polymerase. Repeating this process through multiple cycles amplifies the targeted DNA region. At the start of each cycle, the mixture of template and primers is heated, separating the newly synthesized molecule and template. Then, as the mixture cools, both of these become templates for annealing of new primers, and the polymerase extends from these. As a result, the number of copies of the target region doubles each round, increasing exponentially.
Donald would also face problems resulting from his own voice in Donald's Dream Voice (May 21, 1948). He works as a door-to-door salesman but his customers do not understand a word he is saying. His attempts at politeness are misinterpreted and customers react angrily to imagined insults. But Daisy convinces him otherwise "Don't give up! I have faith in you!" His problems seem to end when Donald buys a box of "voice pills", a medicine temporarily improving his voice. He gets confident enough in his new-found voice to prepare his marriage proposal for Daisy. But due to an accident he loses all but one of his pills. The rest of the short features his frustrated attempt to regain this last pill in order to propose to her. Something which he is eventually unable to do. After a few minutes of trying to get it, the pill ends up getting swallowed by a cow and makes it able to talk. And tells Donald he can't understand what he's saying. Donald then throws a tantrum.
Daisy would not appear again until Crazy Over Daisy (March 24, 1950). The short took place in an 1890s setting. At first, Donald seems in good mood and on his way for his date with Daisy. But when Chip 'n Dale start ridiculing his appearance the short results in one of their typical fights. Interrupted in the end by Daisy herself who accuses Donald of being cruel to the two "innocent" chipmunks. The short ends with Donald having to forget about that date. The short introduced Daisy's theme song "Crazy over Daisy," and in later appearances, Donald can be heard whistling the tune, such as in "Dude Duck" and "Out on a Limb."
Daisy's final appearance in the Golden Age of American animation was in Donald's Diary (March 5, 1954). There she played the role of a young lady who manages to start a long-term relationship with Donald. But after having a nightmare about the anxieties that would come from married life, Donald runs out on her and joins the French Foreign Legion. Several scenes of the short imply that Daisy has had several previous relationships with men. Donald carves their names on a tree. Not noticing than the opposing side of the tree features her name alongside that of several other boyfriends. The marriage scene in Donald's dream featured a group of sailors waving goodbye to Daisy and mourning the loss of their apparent lover. The story bore little continuity with the "real" Donald and Daisy as Huey, Dewey, and Louie appeared as Daisy's younger brothers. Even still, it was the only time in which Daisy's parents are seen.